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Advantages of TB Diagnostics through Culture methods are as follows:

Disposal of biohazardous waste is essential to prevent contamination and possible infection of those handling the infected specimens.

At the end of each day, Cartridge-based Nucleic Acid Amplification Test (CBNAAT) laboratories generate contaminated material such as:

• Used sputum containers
• Used cartridges
• Transfer pipettes.

All used materials should be considered contaminated.

It is essential to follow biosafety protocols while handling specimens and cartridges. This will prevent anyone handling the specimens and cartridges from getting infected.

General Biosafety Requirements for CBNAAT

Cartridge-based Nucleic Acid Amplification Test (CBNAAT) is a low-risk procedure and requires the same level of precautions, like those used for direct Acid-fast Bacillus (AFB) sputum smear microscopy:

Monitoring of quality indicators is an essential component of quality assurance for the Cartridge-based Nucleic Acid Amplification Test (CBNAAT).

The annual maintenance protocol for the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) instrument involves calibration of the machine. 

Calibration:

It is important to adhere to the manufacturer's installation guide for optimal performance of the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine. This guide should be kept in the laboratory. It is important to thoroughly read the instalment steps given in the brochure supplied with the machine.

Strict monitoring of these criteria should be carried out by the laboratory personnel:

Maintenance of the CBNAAT instrument is an essential activity to be performed in the laboratory setting and involves:

Preventive Maintenance Tasks

• Performed regularly by the user
• Ensure the good performance of the system
• Avoid problems of malfunction

Need-based Maintenance Tasks

• Performed in specific situations
• Performed or guided by manufacturer representatives

Frequency-based Tasks for the CBNAAT Instrument

Some Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine errors will indicate test failure without Error Codes.

Error: Invalid

Problem: 

Sample Processing Control (SPC) failed.

Origin: 

Polymerase Chain Reaction (PCR) was inhibited due to food particles or blood in the sample.

Solution:  

Laboratory Technician (LT) needs to collect another specimen, if necessary.

Prevention: 

Error - 2014, 3074, 3075 and 1001, in the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine are related to ‘Heating Component Related Issues’.

Problem:

Temperature/ Heater Failure

Origins:

• High temperature in module 
• Heater component failure 
• Broken fan 
• Dust on filter near the fan

Troubleshooting:

In the CBNAAT Dx System window:

During the use of the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine, Error- 2008 and 2009 may appear on the screen and needs troubleshooting.

Problem:

2008: Pressure reading exceeds the maximum

2009: Syringe pressure is below the protocol limit

Figure: Error 2008

Origins: