Base Technical Content in text and simple images.
The MGIT 960 is a sensitive instrument to be operated by trained and competent staff. To carry out the liquid culture using this instrument, following steps need to be performed.
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Step 1
To load the tubes, open the desired drawer.
Once the drawer is open, select the desired workflow on the LCD display, in this case, Tube Entry. |
Smear microscopy and culture play an important role in monitoring the response to TB treatment. In NTEP, smear microscopy and culture has been prescribed to monitor the treatment response and at specific time points based on different treatment regimens. The programme also explains the actions that need to be taken based on the follow-up results.
Monitoring of tuberculosis treatment is shown in the table below.
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TYPE OF TREATMENT |
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The disposable, single-use CBNAAT cartridge holds the samples and reagents to be processed in the CBNAAT System. The main cartridge components are described below, and shown in the figure below.
The CBNAAT module contains components that enable automated sample processing in the cartridge, and filling of the tube with the sample-reagent mixture for Polymerase Chain Reaction (PCR), followed by PCR amplification and detection.
The structure of the CBNAAT module is described below and shown in Figure 1:
For a fire to start, three components of the triangle namely: Fuel, Oxygen and Ignition sources need to come together (Figure).
Figure: Components that cause a fire on coming together
There are different types of accidents that can occur in the TB lab. These accidents and actions to be taken are elaborated below:
Tube Breaks or Infectious Spills inside the Biosafety Cabinet (BSC)
The emergency preparedness plan in a tuberculosis (TB) laboratory is a written document that should provide:
Figure: Composition of an Emergency Preparedness Plan
Depending on the procedural risk in the TB lab, the lab is classified into low, moderate and high-risk categories.
Low-risk TB laboratories work on sputum specimens for:
Figure 1: Procedures Conducted in a Low-Risk TB Lab
As part of the biosafety measures in TB labs, there are specific standards that need to be followed for lab equipment.
Key Features of Laboratory Equipment in TB Laboratories
The equipment should be:
Ventilation is an essential biosafety design feature in a TB lab.
Laboratory ventilation has three basic elements:
1. Ventilation rate
Amount of outdoor air that flows into the laboratory.
2. Airflow direction
Overall direction of air flowing through the laboratory should be from functionally clean areas to dirty areas.
3. Airflow pattern