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  • Pyrazinamide PZA Susceptibility Testing

    Content

    The BACTEC MGIT 960 PZA medium tube contains 7 ml of broth. It consists of 5.9 g of modified Middlebrook 7H9 broth and 1.25 Casein peptone per litre of purified water adjusted to pH 5.9.

     

    The BACTEC MGIT PZA Drug Kit contains two vials of 20,000 µg of lyophilized PZA and 6 vials of PZA supplement. Each vial of the supplement contains 15 ml of enrichment with the following formula per litre of water.

     

    Bovine albumin---------------------------50.0 g

    Dextrose -----------------------------------20.0 g

    Polyoxyethyline state (POES) -----------1.1 g

    Catalase -------------------------------------0.03 g

    Olecic acid --------------------------------- 0.1 g

     

    Storage instructions: PZA medium may be stored at 2-25ºC. Do not freeze, avoid exposure to light and do not use if found turbid.

     

    Upon receipt, PZA drug vials should be stored at 2-8ºC. Once reconstituted, the antibiotic solution may be kept frozen at -20ºC or colder for up to six months but should not exceed the original expiration date. Once thawed, do not store or refreeze.

     

    Resource

     

    Mycobacteriology Laboratory Manual   

    Assessment

    Question​

    Answer 1​

    Answer 2​

    Answer 3​

    Answer 4​

    Correct answer​

    Correct explanation​

    Page id​

    Part of Pre-test​

    Part of Post-te
     PZA supplement contains which of the following? Bovine albumin Dextrose Polyoxyethylene state (POES)  All of the above Answer 4 PZA supplement contains Bovine albumin, Dextrose, Polyoxyethylene state (POES), Catalase, and Oleic acid.      

     

  • Critical Concentration and Reconstitution of PZA

    Content

    Critical Concentration for PZA Drugs Recommended for the Treatment of DS- TB

     

    Anti TB drugs

    CC (ug/ml) for DST medium

    Pyrazinamide

    100μg/mL

     

     

     

    Reconstitution of PZA Lyophilized Drugs (Drug stock preparation)

    Reconstitute the PZA drug vial with 2.5 ml sterile distilled/ deionized water to make a stock solution of 8000 μg/ml. Failure to use the appropriate volume will invalidate these tests.

    Drug lyophilised

     

    Volume

    added

     

    Concentration of

    reconstituted agent

    μg/mL

     

    Volume added

    to each MGIT tube

     

    Final concentration

    in MGIT tubes

     

    Pyrazinamide

    2.5ml

    8000 μg/ml

    100

    100μg/mL

     

    Resource

    Mycobacteriology Laboratory Manual      

    Assessment

    Question​

    Answer 1​

    Answer 2​

    Answer 3​

    Answer 4​

    Correct answer​

    Correct explanation​

    Page id​

    Part of Pre-test​

    Part of Post-te

    Which of the following is the Critical Concentration for PZA drugs recommended for the treatment of DS- TB?

     

    		 100μg/mL 10μg/mL 1000μg/mL 800μg/mL Answer 1 Critical Concentration for PZA drugs recommended for the treatment of DS- TB is:100μg/mL.