Prevention of False Negative Results During Smear Microscopy

Content

To prevent false negative results during smear microscopy, each laboratory technician needs to:

Make sure that the sample contains sputum, not just saliva.
Make sure there is enough sputum (at least 2-5 ml).
Select thick, purulent particles to make the smear.
Prepare smears correctly - not too thick, not too thin or not too little material.
Fix the slide for the correct length of the time, not too short or too long.
Stain with Carbol fuchsin for full five minutes.
Do not decolorize with Sulphuric acid too intensively.
Examine every smear for at least five minutes and full 100 fields before recording it as negative.
Label the sputum containers, slides and laboratory forms carefully.
Cross check the number on the laboratory form and sputum container before recording.
Record and report the results accurately.

Resources

Kindly provide your valuable feedback on the page to the link provided HERE