Under Development

 MTB isolates from all positive cultures (using the LJ subculture from an

Maintenance and Storage of Mycobacterial Strains

Catalase Peroxidase test for MTB

Principle:

Principle:

Principle:

PNB p-Nitrobenzoic acid susceptibility of MTB

Principle:

Biochemical Tests for MTB

An experienced laboratory technologist may make the presumptive diagnosis of tuberculosis on the basis of the morphological characteristics of tubercle bacilli, but it is best to do confirmatory tests.

A positive and a negative control must be tested with each new lot or a new shipment of kits received and with each new batch of extraction buffer prepared. Similarly, these controls must be run weekly or along with each batch of patient isolates when tests are set up less frequently.

1. Frequency:

The SD Bioline is a solid-phase immunochromatographic assay that is inexpensive, easy to use and readily available. They are easily stored at room temperature and allow the results within 15 -20 minutes.

Specimen preparation and subsequent steps must be performed in a BSC using full PPE. 

From positive MGIT tubes: