Inoculation Procedure for Storage of Culture Isolates
Steps to Preserve and Store MTB Cultures
Quality Control of Selective Middlebrook- 7H9 Liquid Medium
Quality control (QC) Testing of 7H9 Liquid Medium
Every new lot of MGIT medium and every new lot of the enrichment should be quality control tested by the user upon receipt and before it is used routinely.
A. QC strains
Cultures: The following three mycobacterial cultures are recommended for quality control testing.
1.M. tuberculosis, H37Rv ATCC 27294
2.M. kansasii ATCC 12478
3.M. fortuitum ATCC 6841
Preparation of Selective Middlebrook- 7H9 Liquid Medium
Liquid media are used in the mycobacteriology laboratory for sub-culturing stock strains for storing strains at - 80°C and other in-vitro tests.
Media and consumables required to store culture isolates
MTB isolates from all positive cultures (using the LJ subculture from an
Storage of Culture Isolates
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Maintenance and Storage of Mycobacterial Strains
Catalase peroxidase test for MTB
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Catalase Peroxidase test for MTB
Principle:
Nitrate reduction test for MTB
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Principle:
Niacin production test for MTB
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Principle:
Susceptibility of MTB to p-Nitrobenzoic acid (PNB)
PNB p-Nitrobenzoic acid susceptibility of MTB
Principle:
Biochemical Tests for MTB
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Biochemical Tests for MTB
An experienced laboratory technologist may make the presumptive diagnosis of tuberculosis on the basis of the morphological characteristics of tubercle bacilli, but it is best to do confirmatory tests.