Node Status
Live
Node Status
Learning Objectives
- Provide an overview of the Truenat platform- An indigenous test rapid molecular test to be used at the point of care. It uses RTPCR as the base molecular technology.
- Discuss the important advantages of the platform.
- Rapid test
- Point of care and battery operated
- The type of Kit used would determine the test/ assay that would be performed.
- Discuss the parts of a Truenat machine and the various configurations it comes in.
- Mention that they can identify the nearest Truenat laboratory from Nikshay Directory or the Nikshay Arogya Saathi application.
Sub Nodes
- Intended Use of TrueNAT MTB and TrueNAT MTB Rif Dx Assay
- Truenat Cartridge and Inside Demonstration
- Inbuilt Controls used in TrueNAT
- TrueNAT Test Sample Storage and Transportation Procedure
- DNA Extraction in TrueNAT MTB and TrueNAT MTB/RIF DX Assay
- TrueNAT Sample Processing: Other Body Fluids
- Amplification and Detection of MTB and MTB-Rif
- Truenat MTB Assay Results Interpretation: Visualization
- TrueNAT MTB Assay Results Interpretation: Detection of Mycobacterium tuberculosis and RIF Resistance
- Results Interpretation of Mycobacterium tuberculosis Detected-RIF Indeterminate in TrueNAT Assay
- Limitations of TrueNAT MTB Assay
- Maintenance of the Truenat Instrument - General Principles
- Record Keeping of TrueNAT Instrument Maintenance
- External Quality Assurance of TrueNAT
- Biosafety measures required for Truenat
- Disposal of Infectious Samples and Used Cartridges in TrueNAT Laboratories
- Specific Performance Characteristics of TrueNAT: Analytical Exclusivity [Primer Specificity]
- Interfering Substances causing errors in NAAT Assay
- Daily, Weekly and Monthly Maintenance of Truenat Instrument
- NAAT Instrument Monthly Data Archive and Data Back-up
- Ensuring Compliance of Truenat Instrument Installation Criteria
- Consumables Required at a Truenat Lab
- Overview of the Truenat Testing Process
- Processing of Sputum Sample before Truenat Testing
- Annual Maintenance of the Truenat Instrument
- Monitoring Quality Indicators of Truenat
- Truenat MTB and RIF Assay as a TB Diagnostic Test
- Truenat result interpretation and recording
- Troubleshooting in Truenat
- Truenat Instrument maintenance and recordkeeping
- Biomedical Waste Management in Truenat Lab
- Monitoring Truenat quality and lab performance
- Calibration of Truenat
- Loading the sample into Truenat Cartridge
- Unloading the Truenat Cartridge
- Using Truenat autoanalyzer for MTB testing
- Loading the elute onto the Truenat MTB Chip
- Retrieving test results from the Truenat machine
- Recording Truenat Results